잠시만 기다려 주세요. 로딩중입니다.

Isolation of a Lipolytic and Proteolytic Bacillus licheniformis from Refinery Oily Sludge and Optimization of Culture Conditions for Production of the Enzymes

Microbiology and Biotechnology Letters 2020년 48권 4호 p.515 ~ 524
Devi Sashi Prava, Jha Dhruva Kumar,
소속 상세정보
 ( Devi Sashi Prava ) - Gauhati University Department of Botany
 ( Jha Dhruva Kumar ) - Gauhati University Department of Botany

Abstract


With the increasing demand for enzymes in industrial applications there is a growing need to easily produce industrially important microbial enzymes. This study was carried out to screen the indigenous refinery bacterial isolates for their production of two industrially important enzymes i.e. lipase and protease. A total of 15 bacterial strains were isolated using Soil Extract Agar media from the oil-contaminated environment and one was shown to produce high quality lipase and protease enzymes. The culture conditions (culture duration, temperature, source of nitrogen, carbon, and pH) were optimized to produce the optimum amount of both the lipase (37.6 ± 0.2 Uml-1) and the protease (41 ± 0.4 Uml-1) from this isolate. Productivity of both enzymes was shown to be maximized at pH 7.5 in a medium containing yeast extract and peptone as nitrogen sources and sucrose and galactose as carbon sources when incubated at 35 ± 1℃ for 48 h. Bacterial strain SAB06 was identified as Bacillus licheniformis (MT250345) based on biochemical, morphological, and molecular characteristics. Further studies are required to evaluate and optimize the purification and characterization of these enzymes before they can be recommended for industrial or environmental applications.

키워드

Bacillus licheniformis; refinery sludge; enzymes; optimization; cultural conditions.; Lipase

원문 및 링크아웃 정보

등재저널 정보