잠시만 기다려 주세요. 로딩중입니다.

Voiding Dysfunction in Old Male Rats Associated With Enlarged Prostate and Irregular Afferent-Triggered Reflex Responses

International Neurourology Journal 2020년 24권 3호 p.258 ~ 269
Zhang Chuan, Li Xuhong, Boone Timothy B., Cruz Yolanda, Zhang Yingchun, Munoz Alvaro,
소속 상세정보
 ( Zhang Chuan ) - University of Houston Department of Biomedical Engineering
 ( Li Xuhong ) - Central South University Third Xiangya Hospital
 ( Boone Timothy B. ) - Houston Methodist Hospital Department of Urology
 ( Cruz Yolanda ) - Universidad Autonoma de Tlaxcala Centro Tlaxcala de Biologia de la Conducta
 ( Zhang Yingchun ) - University of Houston Department of Biomedical Engineering
 ( Munoz Alvaro ) - Houston Methodist Research Institute Regenerative Medicine and Tissue Engineering Program-Urology

Abstract


Purpose: This study was conducted to evaluate the hypothesis that an enlarged prostate in old rats may lead to complications associated with voiding dysfunction involving ionotropic P2X2/3-type purinergic receptors

Methods: Intact animals were divided into male young (MYR; 8?10 weeks old) and male old (MOR; 20 months old) rats. The animals underwent simultaneous detrusor electromyography (EMG) and suprapubic cystometry (CMG) under urethane anesthesia. Immunofluorescence techniques were used to evaluate prostatic autonomic innervation and P2X3R expression in bladder urothelial cells. The functional role of P2X3R was characterized by intramuscular application of AF-353, a selective P2X2/3R antagonist.

Results: The prostate index significantly increased in MOR, suggestive of an enlarged prostate affecting micturition patterns. Significant EMG and CMG differences were found between MYR and MOR. Higher immunoreactivity for P2X2/3R in the urothelial layer and for prostatic neurofilaments was seen in MOR. Systemic inhibition of P2X2/3R had minimal effects on MYR responsiveness, but improved voiding function in MOR with a marked decrease of intravesical pressure and bladder contractile responses.

Conclusions: The data support the hypothesis that an enlarged prostate in MOR may contribute to voiding dysfunction involving activation of P2X2/3R, which enhances a prostate-bladder reflex. This reflex may increase bladder afferent transmission and activation of increased prostate innervation, leading to voiding dysfunction.

키워드

Prostate; Urinary bladder; Urothelium; Receptors, Purinergic P2X3; Micturition

원문 및 링크아웃 정보

 

등재저널 정보