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Mechanical Stimulation and Diameter of Fiber Scaffolds Affect the Differentiation of Rabbit Annulus Fibrous Stem Cells

Tissue Engineering and Regenerative Medicine 2021년 18권 1호 p.49 ~ 60
Zhou Pinghui, Wei Bangguo, Guan Jingjing, Chen Yu, Zhu Yansong, Ye Yuchen, Meng Yue, Guan Jianzhong, Mao Yingji,
소속 상세정보
 ( Zhou Pinghui ) - Bengbu Medical College First Affiliated Hospital Department of Orthopedics
 ( Wei Bangguo ) - Bengbu Medical College First Affiliated Hospital Department of Orthopedics
 ( Guan Jingjing ) - Bengbu Medical College First Affiliated Hospital Department of Orthopedics
 ( Chen Yu ) - Bengbu Medical College Anhui Province Key Laboratory of Tissue Transplantation
 ( Zhu Yansong ) - Bengbu Medical College School of Life Sciences
 ( Ye Yuchen ) - Bengbu Medical College First Affiliated Hospital Department of Orthopedics
 ( Meng Yue ) - Bengbu Medical College First Affiliated Hospital Department of Orthopedics
 ( Guan Jianzhong ) - Bengbu Medical College First Affiliated Hospital Department of Orthopedics
 ( Mao Yingji ) - Bengbu Medical College First Affiliated Hospital Department of Orthopedics

Abstract


BACKGROUND: Degeneration of the annulus fibrosus (AF), an important structure of the intervertebral disc, is one of the main causes of degenerative disc disease. Fabrication of scaffolds replicating the stratified microstructure of the AF is critical for the successful regeneration of AF.

METHODS: In this study, we cultured rabbit AF-derived stem cells (AFSCs) using fabricated electrospun fibrous poly-L-lactic acid scaffolds with different diameters. We applied cyclic tensile strain (CTS) on the scaffolds to regulate the differentiation of AFSCs into specific cell types that resided at the inner, middle, and outer zones of the AF.

RESULTS: We found that the morphologies of AFSCs on the smaller-fiber-diameter scaffolds were nearly round, whereas spindle-like cells morphologies were observed on large-diameter scaffolds. CTS enhanced these phenomena and made the cells slender. The expression levels of collagen-I in cells increased as a function of the fiber diameter, whereas collagen-II and aggrecan exhibited opposite trends. Moreover, the application of CTS upregulated the gene expressions of collagen-I, collagen-II, and aggrecan.

CONCLUSION: Overlaying the scaffolds with different CTS-stimulated cells could eventually lead to engineered AF tissues with hierarchical structures that approximated the native AF tissue. Thus, the proposed methodologies could be potentially applied for AF regeneration.

키워드

Intervertebral disc degeneration; Cyclic tensile strain; Poly-L-lactic acid scaffold; Annulus fibrosus-derived stem cells; Differentiation

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