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Novel Marine Organism-Derived Extracellular Vesicles for Control of Anti-Inflammation

Tissue Engineering and Regenerative Medicine 2021년 18권 1호 p.71 ~ 79
조성한, 김창수, 박상혁,
소속 상세정보
조성한 ( Jo Sung-Han ) - Pukyong National University Industry 4.0 Convergence Bionics Engineering
김창수 ( Kim Chang-Su ) - Kosin University Gospel Hospital Department of Orthopedic Surgery
박상혁 ( Park Sang-Hyug ) - Pukyong National University Department of Biomedical Engineering

Abstract


Background: Extracellular vesicles (EVs) exhibit potential as functional biomolecules for tissue regeneration and immunomodulation as they play important roles in the physiological communication between cells. EV internal cargo contains miRNAs, proteins, lipids, and so on. Osteoarthritis (OA) is a common joint disease causing disability owing to impaired joint function and pain. EVs originating from animal cells and tissue matrices are also being considered for OA, in addition to research involving non-steroidal therapeutic agents. However, there are no studies on EVs from marine organisms. Hence, we focused on sea cucumber-derived EVs and conducted experiments to set up an extraction protocol and to demonstrate their efficacy to modulate the inflammatory environment.

Methods: Sea cucumber extracellular matrices (SECMs) were prepared by a decellularization process. Lyophilized SECMs were treated with collagenase and filtered to isolate sea cucumber extracellular vesicles (SEVs). After isolation, we conducted physical characterization and cell activation studies including cytotoxicity, proliferation, and anti-inflammation effect assays.

Results: The physical characterization results showed circular SEVs in the size range of 66?480 nm. These SEVs contained large amounts of protein cargo, infiltrated the synoviocyte membrane without damage, and had a suppressive effect on inflammatory cytokines.

Conclusion: This study established an extraction process for EVs from sea cucumber and reported the anti-inflammatory ability of SEVs. Isolated SEVs can be further utilized for tissue regeneration studies and can be compared to various marine or animal-derived EVs.

키워드

Extracellular matrix; Extracellular vesicles; Sea cucumber; Marine organism; Anti-inflammation

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