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Investigation of the Clinical Potential of Polarization-Sensitive Optical Coherence Tomography in a Laryngeal Tumor Model

Tissue Engineering and Regenerative Medicine 2021년 18권 1호 p.81 ~ 87
Xin Zhou, 김성원, 옥철호, 권다영, 최진혁, 고택용, 김준형, Tang Shuo, 안예찬,
소속 상세정보
 ( Xin Zhou ) - University of British Columbia Department of Electrical and Computer Engineering
김성원 ( Kim Sung-Won ) - Kosin University College of Medicine Department of Otolaryngology-Head and Neck Surgery
옥철호 ( Oak Chul-Ho ) - Kosin University College of Medicine Department of Internal Medicine
권다영 ( Kwon Daa-Young ) - Pukyong National University Department of Biomedical Engineering
최진혁 ( Choi Jin-Hyuk ) - Kosin University College of Medicine Department of Surgery
고택용 ( Ko Taek-Yong ) - Kosin University College of Medicine Department of Thoracic and Cardiovascular Surgery
김준형 ( Kim Jun-Hyeong ) - Kosin University College of Medicine Department of Internal Medicine
 ( Tang Shuo ) - University of British Columbia Department of Electrical and Computer Engineering
안예찬 ( Ahn Yeh-Chan ) - Pukyong National University Department of Biomedical Engineering

Abstract


BACKGROUND: The vocal cord tissue consists of three anatomical layers from the surface to deep inside: the epithelium that contains almost no collagen, the lamina propria that is composed of abundant collagen, and the vocalis muscle layer. It is clinically important to visualize the tissue microstructure using a non-invasive method, especially in the case of vocal cord nodules or cancer, since histological changes in each layer of the vocal cord cause changes in the voice. Polarization-sensitive optical coherence tomography (PS-OCT) enables phase retardation measurement to evaluate birefringence of tissue with varied organization of collagen fibers in different tissue layers. Therefore, PS-OCT can visualize structural changes between normal and abnormal vocal cord tissue.

METHOD: A rabbit laryngeal tumor model with different stages of tumor progression was investigated ex-vivo by PS-OCT. A phase retardation slope-based analysis, which quantifies the birefringence in different layers, was conducted to distinguish the epithelium, lamina propria, and muscle layers.

RESULTS: The PS-OCT images showed a gradual decrease in birefringence from normal tissue to advanced tumor tissue. The quantitative analysis provided a more detailed comparison among different stages of the rabbit laryngeal tumor model, which was validated by the corresponding histological findings.

CONCLUSION: Differences in tissue birefringence was evaluated by PS-OCT phase retardation measurement. It is also possible to indirectly infer the dysplastic changes based on the mucosal and submucosal alterations.

키워드

Biomedical engineering; Optical tomography; Laryngeal cancer; Birefringence; Collagen

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