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Hyaluronic Acid Coating on Hydrophobic Tracheal Scaffold Enhances Mesenchymal Stem Cell Adhesion and Tracheal Regeneration

Tissue Engineering and Regenerative Medicine 2021년 18권 2호 p.225 ~ 233
Choi Ji-Suk, 이민상, 김주영, 엄민례, 정은지, 이민형, 박수아, 정지훈, 권성근,
소속 상세정보
 ( Choi Ji-Suk ) - Seoul National University Hospital Department of Otorhinolaryngology-Head and Neck Surgery
이민상 ( Lee Min-Sang ) - Sungkyunkwan University School of Pharmacy
김주영 ( Kim Joo-Young ) - Seoul National University Hospital Department of Otorhinolaryngology-Head and Neck Surgery
엄민례 ( Eom Min-Rye ) - Seoul National University Hospital Department of Otorhinolaryngology-Head and Neck Surgery
정은지 ( Jeong Eun-Ji ) - Seoul National University Hospital Department of Otorhinolaryngology-Head and Neck Surgery
이민형 ( Lee Min-Hyung ) - Seoul National University College of Medicine Department of Otorhinolaryngology-Head and Neck Surgery
박수아 ( Park Su-A ) - Korea Institute of Machinery and Materials Department of Nature-Inspired Nanoconvergence Systems
정지훈 ( Jeong Ji-Hoon ) - Sungkyunkwan University School of Pharmacy
권성근 ( Kwon Seong-Keun ) - Seoul National University Hospital Department of Otorhinolaryngology-Head and Neck Surgery

Abstract


BACKGROUND: Long segmental tracheal repair is challenging in regenerative medicine due to low adhesion of stem cells to tracheal scaffolds. Optimal transplantation of stem cells for tracheal defects has not been established. We evaluated the role of hyaluronic acid (HA) coating of tracheal scaffolds in mesenchymal stem cell (MSC) adhesion and tracheal regeneration in a rabbit model.

METHODS: A three-dimensionally printed tubular tracheal prosthesis was incubated with dopa-HA-fluorescein isothiocyanate in phosphate-buffered saline for 2 days. MSCs were incubated with an HA-coated scaffold, and their adhesion was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. HA coated scaffolds with or without MSC seeding were transplanted at the circumferential tracheal defect in rabbits, and survival, rigid bronchoscopy, radiologic findings, and histologic findings were compared between the two groups.

RESULTS: HA-coated scaffolds showed better MSC adhesion than non-coated scaffolds. The HA-coated scaffolds with MSC group showed a wider airway and greater mucosal regeneration compared to the HA-coated scaffolds without MSC group.

CONCLUSION: HA coating of scaffolds can promote MSC adhesion and tracheal regeneration.

키워드

Trachea regeneration; Mesenchymal stem cell; Hyaluronic acid

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