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Effect of PDGF-B Gene-Activated Acellular Matrix and Mesenchymal Stem Cell Transplantation on Full Thickness Skin Burn Wound in Rat Model

Tissue Engineering and Regenerative Medicine 2021년 18권 2호 p.235 ~ 251
Paramasivam Tamilmahan, Maiti Swapan Kumar, Palakkara Sangeetha, Rashmi, Mohan Divya, Manjunthaachar H. V., Karthik K., Kumar Naveen,
소속 상세정보
 ( Paramasivam Tamilmahan ) - ICAR-Indian Veterinary Research Institute Division of Veterinary Surgery
 ( Maiti Swapan Kumar ) - ICAR-Indian Veterinary Research Institute Division of Veterinary Surgery
 ( Palakkara Sangeetha ) - ICAR-Indian Veterinary Research Institute Division of Veterinary Surgery
 ( Rashmi ) - ICAR-Indian Veterinary Research Institute Division of Veterinary Surgery
 ( Mohan Divya ) - ICAR-Indian Veterinary Research Institute Division of Veterinary Surgery
 ( Manjunthaachar H. V. ) - ICAR-Indian Veterinary Research Institute Division of Veterinary Parasitology
 ( Karthik K. ) - ICAR-Indian Veterinary Research Institute Division of Veterinary Microbiology
 ( Kumar Naveen ) - ICAR-Indian Veterinary Research Institute Division of Veterinary Surgery

Abstract


Background: Full thickness burn wounds are lack of angiogenesis, cell migration, epithelialisation and finally scar tissue formation. Tissue engineered composite graft can provide sustained release of growth factor and promote the wound healing by cell migration, early angiogenesis and proliferation of extracellular matrix and wound remodeling. The objective of this study was to evaluate the gene embedded (pDNA-platelet-derived growth factor, PDGF-B) porcine acellular urinary bladder matrix with transfected mesenchymal stem cells (rBMSC) on healing of full thickness burn wound in rat model.

Methods: Full thickness burn wound of 2?×?2 cm size was created in dorsum of rat model under general anesthesia. Burn wounds were treated with silver sulfadiazine; porcine acellular urinary bladder matrix (PAUBM); PAUBM transfected with pDNA-PDGF-B; PAUBM seeded with rBMSC; PAUBM seeded with rBMSC transfected with pDNA-PDGF-B in groups A, B, C, D and E respectively. The wound healing was assessed based on clinical, macroscopically, immunologically, histopathological and RT-qPCR parameters.

Results: Wound was significantly healed in group E and group D with early extracellular matrix deposition, enhanced granulation tissue formation and early angiogenesis compared to all other groups. The immunologic response against porcine acellular matrix showed that PDGF-B gene activated matrix along with stem cell group showed less antibody titer against acellular matrix than other groups in all intervals. PDGF gene activated matrix releasing the PDGF-B and promote the healing of full thickness burn wound with neovascularization and neo tissue formation. PDGF gene also enhances secretion of other growth factors results in PDGF mediated regenerative activities. This was confirmed in RT-qPCR at various time intervals.

Conclusion: Gene activated matrix encoded for PDGF-B protein transfected stem cells have been clinically proven for early acceleration of angiogenesis and tissue regeneration in burn wounds in rat models.

키워드

Acellular matrix; PDGF-B; Gene therapy; Stem cell; Burn wound

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